A selective medium for Pasteurella multocida and its use with animal and human specimens.

نویسندگان

  • D P Knight
  • J E Paine
  • D C Speller
چکیده

A selective medium (CGT medium), containing clindamycin, gentamicin, potassium tellurite and amphotericin B in 5% horse-blood agar, allowed unimpaired growth of almost all strains of Pasteurella multocida, and P pneumotropica, while inhibiting other bacteria that might be encountered in upper respiratory tract secretions. With its use, P multocida was readily detected in oral swabs from four of 23 dogs, and 10 of 25 cats, but not detected in oral swabs from 47 human subjects. One of 500 sputum specimens yielded P pneumotropica.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Selective medium for Pasteurella multocida and its use to detect oropharyngeal carriage in pig breeders.

A selective culture medium for isolation of Pasteurella multocida was prepared by incorporating 2 mg of amikacin per liter, 4 mg of vancomycin per liter, and 4 mg of amphotericin B per liter into Mueller-Hinton blood agar. Use of this medium revealed the presence of P. multocida in the oropharynges of 19 of the 49 pig breeders who were examined.

متن کامل

Isolation of pasteurella multocida from cows and buffaloes in Urmia's Slaughter House

Pasteurellosis is one of the important economic diseases in ruminants, especially in cows and buffaloes. It is caused by Pasteurella multocida and occasionally by Mannheimia haemolytica. The aim of this study was to isolate Pasteurella multocida from lungs with probable mentioned bacterial agents in cows and buffaloes in Urmia's slaughter house. 240 lung samples over a period of 12 months were ...

متن کامل

Evaluation of transport media for Pasteurella multocida isolates from rabbit nasal specimens.

A suitable medium for the transport of Pasteurella multocida in nasal specimens from rabbits was investigated by using pure cultures of the organism and nasal swabs from infected rabbits. First, the ability of eight transport media to preserve the viabilities of P. multocida strains isolated from rabbits was studied. Cary-Blair medium and Leibovitz medium no. 15 (L-15) were found to be superior...

متن کامل

A new selective enrichment procedure for isolating Pasteurella multocida from avian and environmental samples.

A selective enrichment procedure, using two new selective media, was developed to isolate Pasteurella multocida from wild birds and environmental samples. These media were developed by testing 15 selective agents with six isolates of P. multocida from wild avian origin and seven other bacteria representing genera frequently found in environmental and avian samples. The resulting media--Pasteure...

متن کامل

Molecular characterization of Pasteurella multocida isolates obtained from poultry, ruminant, cats and dogs using RAPD and REP-PCR analysis

In the present study, Randomly Amplified Polymorphic DNA‌ (RAPD) and Repetitive Extragenic Palindromic sequence-based Polymerase Chain Reaction (REP-PCR) were used to characterize 131 isolates of Pasteurella multocida, originating from different healthy and diseased animal species obtained from several geographical regions of Iran. The RAPD and REP-PCR generated amplified products in the range ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of clinical pathology

دوره 36 5  شماره 

صفحات  -

تاریخ انتشار 1983